Mouse anti-Aquaporin 4 Antibody
Product Specifications
Background
Mouse monoclonal antibody to Aquaporin 4
Synonyms
Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Aquaporin 4. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC, IF/IC
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/3000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Aquaporin 4 expression in Hela (A), mouse heart (B), rat heart (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Aquaporin 4 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Aquaporin 4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
361
Gene ID (Mouse)
11829
Gene ID (Rat)
25293
SwissProt (Human)
P55087
SwissProt (Mouse)
P55088
SwissProt (Rat)
P47863
CAS Number
9007-83-4
Available Sizes
Curated Selection
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