Mouse anti-Alpha-tubulin (AcK40) Antibody
Product Specifications
Background
Mouse monoclonal antibody to Alpha-tubulin (AcK40)
Synonyms
Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-tubulin. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Alpha-tubulin (AcK40) expression in HeLa (A) whole cell lysates.
IHC description
Immunohistochemical analysis of Alpha-tubulin (AcK40) staining in human breast cancer, mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
7277
SwissProt (Human)
P68363
CAS Number
9007-83-4
Available Sizes
Curated Selection
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