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Mouse anti-Alpha-actin-1 Antibody

Product Specifications

Background

Mouse monoclonal antibody to Alpha-actin-1

CAS Number

9007-83-4

Synonyms

ACTA; Actin alpha skeletal muscle; Alpha-actin-1

Host

Mouse

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-actin-1. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IP

Concentration

1mg/mL

Dilution

WB (1/5000 - 1/10000), IH (1/200 - 1/500), IP (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of Alpha-actin-1 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Alpha-actin-1 staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description

Immunoprecipitation of Alpha-actin-1 from 0.5mg mouse brain whole cell extract lysate, using anti-Alpha-actin-1 Antibody.

Gene ID (Human)

58

Gene ID (Mouse)

11459

Gene ID (Rat)

29437

SwissProt (Human)

P68133

SwissProt (Mouse)

P68134

SwissProt (Rat)

P68136

Available Sizes

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