Mouse anti-active Caspase 3 Antibody

Product Specifications

Background

Mouse monoclonal antibody to active Caspase 3

CAS Number

9007-83-4

Synonyms

CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Host

Mouse

Reactivity

H, M, R

Immunogen

Recombinant protein corresponding to human active Caspase 3.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of active Caspase 3 expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of active Caspase 3 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.