Mouse anti-active Caspase 3 Antibody
Product Specifications
Background
Mouse monoclonal antibody to active Caspase 3
Synonyms
CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Host
Mouse
Reactivity
H, M, R
Immunogen
Recombinant protein corresponding to human active Caspase 3.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of active Caspase 3 expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of active Caspase 3 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
836
SwissProt (Human)
P42574
CAS Number
9007-83-4
Available Sizes
Curated Selection
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