What is the catalog number for Rabbit anti-ZIC1/2/3 Antibody?

The catalog number for Rabbit anti-ZIC1/2/3 Antibody is YLD8289.

What sizes are available for Rabbit anti-ZIC1/2/3 Antibody?

Rabbit anti-ZIC1/2/3 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-ZIC1/2/3 Antibody come in?

Rabbit anti-ZIC1/2/3 Antibody is available in 2 different pack sizes.

Is Rabbit anti-ZIC1/2/3 Antibody in stock?

Rabbit anti-ZIC1/2/3 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

Rabbit anti-ZIC1/2/3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ZIC1/2/3

CAS Number

9007-83-4

Synonyms

ZIC1; ZIC; ZNF201; Zinc finger protein ZIC 1; Zinc finger protein 201; Zinc finger protein of the cerebellum 1; ZIC2; Zinc finger protein ZIC 2; Zinc finger protein of the cerebellum 2; ZIC3; ZNF203; Zinc finger protein ZIC 3; Zinc finger protein 203; Zinc finger protein of the cerebellum 3

Host

Rabbit

Reactivity

H, M, R, C

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZIC1/2/3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of ZIC1/2/3 expression in SW480 (A), Jurkat (B), SHSY5Y (C) whole cell lysates.

IHC description

Immunohistochemical analysis of ZIC1/2/3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of ZIC1/2/3 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.