Rabbit anti-YT521-B Antibody
Product Specifications
Background
Rabbit polyclonal antibody to YT521-B
Synonyms
KIAA1966; YT521; YTH domain-containing protein 1; Putative splicing factor YT521; YT521-B
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human YT521-B
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of YT521-B expression in MCF7 (A), Jurkat (B), mouse brain (C), rat brain (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of YT521-B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
91746
Gene ID (Mouse)
231386
Gene ID (Rat)
170956
SwissProt (Human)
Q96MU7
SwissProt (Mouse)
E9Q5K9
SwissProt (Rat)
Q9QY02
CAS Number
9007-83-4
Available Sizes
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