Rabbit anti-Wilms Tumor 1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Wilms Tumor 1
CAS Number
9007-83-4
Synonyms
Wilms tumor protein; WT33
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Wilms Tumor 1
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of Wilms Tumor 1 expression in A549 (A), MCF7 (B), mouse heart (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Wilms Tumor 1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Wilms Tumor 1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
7490
Gene ID (Rat)
24883
SwissProt (Human)
P19544
SwissProt (Mouse)
P22561
SwissProt (Rat)
P49952
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC040029-100 1x 100 µL
Fibronectin (TV-1), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC400696-100 1x 100 µL
Cytokeratin 10/13 (DE-K13), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC041365-500 1x 500 µL
Adipophilin / Perilipin-2 (Marker of Obesity) (ADFP/1365), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC401188-500 1x 500 µL
SUMO-1 (SUMO1/1188), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details