Rabbit anti-WIF-1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to WIF-1
Synonyms
Wnt inhibitory factor 1; WIF-1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human WIF-1
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of WIF-1 expression in mouse heart (A), mouse skeletal muscle (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of WIF-1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
11197
Gene ID (Mouse)
24117
Gene ID (Rat)
114557
SwissProt (Human)
Q9Y5W5
SwissProt (Mouse)
Q9WUA1
SwissProt (Rat)
Q6IN38
CAS Number
9007-83-4
Available Sizes
Curated Selection
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