Rabbit anti-Vinculin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Vinculin
Synonyms
Vinculin; Metavinculin; MV
Host
Rabbit
Reactivity
H, M, R, C, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Vinculin expression in MCF7 (A), HepG2 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of Vinculin staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Vinculin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
7414
Gene ID (Mouse)
22330
Gene ID (Rat)
305679
SwissProt (Human)
P18206
SwissProt (Mouse)
Q64727
SwissProt (Rat)
P85972
CAS Number
9007-83-4
Available Sizes
Curated Selection
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