Rabbit anti-UCH-L3 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to UCH-L3
Synonyms
Ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3; Ubiquitin thioesterase L3
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human UCH-L3
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of UCH-L3 expression in SHSY5Y (A), HT29 (B), mouse testis (C), rat testis (D) whole cell lysates.
IHC description
Immunohistochemical analysis of UCH-L3 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
7347
Gene ID (Mouse)
50933
Gene ID (Rat)
498560
SwissProt (Human)
P15374
SwissProt (Mouse)
Q9JKB1
SwissProt (Rat)
Q91Y78
CAS Number
9007-83-4
Available Sizes
Curated Selection
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