Rabbit anti-UBE2B Antibody
Product Specifications
Background
Rabbit polyclonal antibody to UBE2B
Synonyms
RAD6B; Ubiquitin-conjugating enzyme E2 B; RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human UBE2B
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of UBE2B expression in Jurkat (A), NIH3T3 (B), mouse heart (C), rat heart (D), rat brain (E) whole cell lysates.
IHC description
Immunohistochemical analysis of UBE2B staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
7320
Gene ID (Mouse)
22210
Gene ID (Rat)
81816
SwissProt (Human)
P63146
SwissProt (Mouse)
P63147
SwissProt (Rat)
P63149
CAS Number
9007-83-4
Available Sizes
Curated Selection
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