Rabbit anti-UBE2B Antibody

Product Specifications

Background

Rabbit polyclonal antibody to UBE2B

CAS Number

9007-83-4

Synonyms

RAD6B; Ubiquitin-conjugating enzyme E2 B; RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human UBE2B

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of UBE2B expression in Jurkat (A), NIH3T3 (B), mouse heart (C), rat heart (D), rat brain (E) whole cell lysates.

IHC description

Immunohistochemical analysis of UBE2B staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.