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Rabbit anti-Ub Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Ub

Synonyms

UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A; UBA80; UBCEP1; Ubiquitin-40S ribosomal protein S27a; Ubiquitin carboxyl extension protein 80; UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1

Host

Rabbit

Reactivity

H, M, R, B, C, Mk, P, Rb

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ub. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Ub expression in HeLa (A), NIH3T3 (B), rat kidney (C), HL60 (D) whole cell lysates.

IHC description

Immunohistochemical analysis of Ub staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

7311

Gene ID (Mouse)

22187; 22190; 78294; 22186

Gene ID (Rat)

192255; 50522; 100912032; 64156

SwissProt (Human)

P0CG47; P0CG48; P62979; P62987

SwissProt (Mouse)

P0CG49; P0CG50; P62983; P62984

SwissProt (Rat)

P0CG51; Q63429; P62982; P62986

CAS Number

9007-83-4

Available Sizes

Curated Selection

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