Rabbit anti-U2AF1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to U2AF1
Synonyms
U2AF35; U2AFBP; Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human U2AF1
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of U2AF1 expression in A549 (A), MCF7 (B), mouse pancreas (C), rat liver (D) whole cell lysates.
IHC description
Immunohistochemical analysis of U2AF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
102724594; 7307
Gene ID (Mouse)
108121
SwissProt (Human)
Q01081
SwissProt (Mouse)
Q9D883
CAS Number
9007-83-4
Available Sizes
Curated Selection
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