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Rabbit anti-Tryptase alpha/beta Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Tryptase alpha/beta

CAS Number

9007-83-4

Synonyms

TPS1; TPS2; TPSB1; Tryptase alpha/beta-1; Tryptase-1; Tryptase I; Tryptase alpha-1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Tryptase alpha/beta

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of Tryptase alpha/beta expression in HepG2 (A), mouse brain (B), mouse liver (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of Tryptase alpha/beta staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

7177

Gene ID (Mouse)

100503895

Gene ID (Rat)

54271

SwissProt (Human)

Q15661

SwissProt (Mouse)

Q02844

SwissProt (Rat)

P27435

Available Sizes

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