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Rabbit anti-TRPV5 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to TRPV5

CAS Number

9007-83-4

Synonyms

ECAC1; Transient receptor potential cation channel subfamily V member 5; TrpV5; Calcium transport protein 2; CaT2; Epithelial calcium channel 1; ECaC; ECaC1; Osm-9-like TRP channel 3; OTRPC3

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human TRPV5

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of TRPV5 expression in HepG2 (A), HT29 (B), mouse kidney (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of TRPV5 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

56302

Gene ID (Mouse)

194352

Gene ID (Rat)

116469

SwissProt (Human)

Q9NQA5

SwissProt (Mouse)

P69744

SwissProt (Rat)

Q9JIP0

Available Sizes

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