Rabbit anti-Tropomyosin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Tropomyosin
Synonyms
C15orf13; TMSA; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Tropomyosin
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Tropomyosin expression in SW480 (A), MCF7 (B), mouse heart (C), mouse brain (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Tropomyosin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
7168
Gene ID (Mouse)
22003
Gene ID (Rat)
24851
SwissProt (Human)
P09493
SwissProt (Mouse)
P58771
SwissProt (Rat)
P04692
CAS Number
9007-83-4
Available Sizes
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