Rabbit anti-Transgelin-2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Transgelin-2
Synonyms
KIAA0120; Transgelin-2; Epididymis tissue protein Li 7e; SM22-alpha homolog
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Transgelin-2
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Transgelin-2 expression in HEK293T (A), Hela (B), THP1 (C), MCF7 (D), SKOV3 (E), mouse lung (F) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Transgelin-2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
8407
Gene ID (Mouse)
21346
Gene ID (Rat)
304983
SwissProt (Human)
P37802
SwissProt (Mouse)
Q9WVA4
SwissProt (Rat)
Q5XFX0
CAS Number
9007-83-4
Available Sizes
Curated Selection
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