Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-TPH1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to TPH1

Synonyms

TPH; TPRH; TRPH; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1

Host

Rabbit

Reactivity

H, M, R, C, Rb

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human TPH1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of TPH1 expression in Jurkat (A), HepG2 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of TPH1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of TPH1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

7166

Gene ID (Mouse)

21990

Gene ID (Rat)

24848

SwissProt (Human)

P17752

SwissProt (Mouse)

P17532

SwissProt (Rat)

P09810

CAS Number

9007-83-4

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Coriandrin
T126304-01 10mg

Coriandrin

Ask
View Details
Coriandrin
T126304-02 1g

Coriandrin

Ask
View Details
Coriandrin
T126304-03 1mg

Coriandrin

Ask
View Details
Coriandrin
T126304-04 50mg

Coriandrin

Ask
View Details
Coriandrin
T126304-05 5mg

Coriandrin

Ask
View Details
Rabbit Polyclonal TNN Antibody [Janelia Fluor 646]
NBP2-98639JF646 0.1 mL

Rabbit Polyclonal TNN Antibody [Janelia Fluor 646]

Ask
View Details