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Rabbit anti-Topoisomerase 3 beta1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Topoisomerase 3 beta1

Synonyms

TOP3B1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1

Host

Rabbit

Reactivity

H, M, R, B, D, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Topoisomerase 3 beta1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Topoisomerase 3 beta1 expression in HepG2 (A), A431 (B), HeLa (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Topoisomerase 3 beta1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

8940

Gene ID (Mouse)

21976

SwissProt (Human)

O95985

SwissProt (Mouse)

Q9Z321

CAS Number

9007-83-4

Available Sizes

Curated Selection

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