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Rabbit anti-Topoisomerase 2 alpha Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Topoisomerase 2 alpha

Synonyms

TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme

Host

Rabbit

Reactivity

H, M, R, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Topoisomerase 2 alpha. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Topoisomerase 2 alpha expression in Hela (A), Jurkat (B), mouse brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Topoisomerase 2 alpha staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

7153

Gene ID (Mouse)

21973

Gene ID (Rat)

360243

SwissProt (Human)

P11388

SwissProt (Mouse)

Q01320

SwissProt (Rat)

P41516

CAS Number

9007-83-4

Available Sizes

Curated Selection

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