Rabbit anti-Topoisomerase 1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Topoisomerase 1
Synonyms
DNA topoisomerase 1; DNA topoisomerase I
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide of human Topoisomerase 1
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Topoisomerase 1 expression in Jurkat (A), Hela (B), mouse spleen (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Topoisomerase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
7150
Gene ID (Mouse)
21969
SwissProt (Human)
P11387
SwissProt (Mouse)
Q04750
CAS Number
9007-83-4
Available Sizes
Curated Selection
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