Rabbit anti-TNF alpha Antibody
Product Specifications
Background
Rabbit polyclonal antibody to TNF alpha
Synonyms
TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant protein corresponding to human TNF alpha.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of TNF alpha expression in HepG2 LPS-treated (A) whole cell lysates.
IHC description
Immunohistochemical analysis of TNF alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
7124
SwissProt (Human)
P01375
CAS Number
9007-83-4
Available Sizes
Curated Selection
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