Rabbit anti-TIM3 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to TIM3
CAS Number
9007-83-4
Synonyms
TIM3; TIMD3; Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide of human TIM3
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of TIM3 expression in HepG2 (A), BT474 (B), mouse lung (C), mouse thymus (D) whole cell lysates.
IHC description
Immunohistochemical analysis of TIM3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
84868
Gene ID (Mouse)
171285
SwissProt (Human)
Q8TDQ0
SwissProt (Mouse)
Q8VIM0
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