Rabbit anti-TIF1 alpha Antibody
Product Specifications
Background
Rabbit polyclonal antibody to TIF1 alpha
Synonyms
RNF82; TIF1; TIF1A; Transcription intermediary factor 1-alpha; TIF1-alpha; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; Tripartite motif-containing protein 24
Host
Rabbit
Reactivity
H, B, D, Mk, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TIF1 alpha. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of TIF1 alpha expression in MCF7 (A), HeLa (B) whole cell lysates.
IHC description
Immunohistochemical analysis of TIF1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
8805
SwissProt (Human)
O15164
CAS Number
9007-83-4
Available Sizes
Curated Selection
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