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Rabbit anti-TGF alpha Antibody

Product Specifications

Background

Rabbit polyclonal antibody to TGF alpha

Synonyms

Protransforming growth factor alpha

Host

Rabbit

Reactivity

H, M, R, B, D, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TGF alpha. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of TGF alpha expression in HEK293T (A), mouse liver (B), mouse kidney (C), rat kidney (D) whole cell lysates.

IHC description

Immunohistochemical analysis of TGF alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

7039

Gene ID (Mouse)

21802

Gene ID (Rat)

24827

SwissProt (Human)

P01135

SwissProt (Mouse)

P48030

SwissProt (Rat)

P01134

CAS Number

9007-83-4

Available Sizes

Curated Selection

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