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Rabbit anti-TAP2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to TAP2

Synonyms

ABCB3; PSF2; RING11; Y1; Antigen peptide transporter 2; APT2; ATP-binding cassette sub-family B member 3; Peptide supply factor 2; Peptide transporter PSF2; PSF-2; Peptide transporter TAP2; Peptide transporter involved in antigen processing 2; Really interesting new gene 11 protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human TAP2

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of TAP2 expression in MCF7 (A), mouse spleen (B), mouse lung (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of TAP2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

6891

Gene ID (Mouse)

21355

Gene ID (Rat)

24812

SwissProt (Human)

Q03519

SwissProt (Mouse)

P36371

SwissProt (Rat)

P36372

CAS Number

9007-83-4

Available Sizes

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