Rabbit anti-Syntenin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Syntenin
Synonyms
MDA9; SYCL; Syntenin-1; Melanoma differentiation-associated protein 9; MDA-9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; TACIP18; Scaffold protein Pbp1; Syndecan-binding protein 1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Syntenin
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Syntenin expression in SW480 (A), THP1 (B), Raji (C), mouse liver (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Syntenin staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
6386
Gene ID (Mouse)
53378
Gene ID (Rat)
83841
SwissProt (Human)
O00560
SwissProt (Mouse)
O08992
SwissProt (Rat)
Q9JI92
CAS Number
9007-83-4
Available Sizes
Curated Selection
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