Rabbit anti-SPHK2 (pT614) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SPHK2 (pT614)
Synonyms
Sphingosine kinase 2; SK 2; SPK 2
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SPHK2. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of SPHK2 (pT614) expression in HEK293T (A), HepG2 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of SPHK2 (pT614) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
56848
Gene ID (Mouse)
56632
SwissProt (Human)
Q9NRA0
SwissProt (Mouse)
Q9JIA7
CAS Number
9007-83-4
Available Sizes
Curated Selection
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