Rabbit anti-SMUG1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SMUG1
CAS Number
9007-83-4
Synonyms
Single-strand selective monofunctional uracil DNA glycosylase
Host
Rabbit
Reactivity
H, M, R, R, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMUG1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of SMUG1 expression in HeLa (A), Molt4 (B), Raw264.7 (C), H9C2 (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of SMUG1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
23583
Gene ID (Mouse)
71726
Gene ID (Rat)
315344
SwissProt (Human)
Q53HV7
SwissProt (Mouse)
Q6P5C5
SwissProt (Rat)
Q811Q1
Available Sizes
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