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Rabbit anti-SMUG1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to SMUG1

CAS Number

9007-83-4

Synonyms

Single-strand selective monofunctional uracil DNA glycosylase

Host

Rabbit

Reactivity

H, M, R, R, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMUG1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of SMUG1 expression in HeLa (A), Molt4 (B), Raw264.7 (C), H9C2 (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of SMUG1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

23583

Gene ID (Mouse)

71726

Gene ID (Rat)

315344

SwissProt (Human)

Q53HV7

SwissProt (Mouse)

Q6P5C5

SwissProt (Rat)

Q811Q1

Available Sizes

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