Rabbit anti-Smoothened Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Smoothened
Synonyms
SMOH; Smoothened homolog; SMO; Protein Gx
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Smoothened. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Smoothened expression in HeLa (A) whole cell lysates.
IHC description
Immunohistochemical analysis of Smoothened staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
6608
Gene ID (Mouse)
319757
SwissProt (Human)
Q99835
SwissProt (Mouse)
P56726
CAS Number
9007-83-4
Available Sizes
Curated Selection
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