Rabbit anti-SMARCAD1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SMARCAD1
CAS Number
9007-83-4
Synonyms
KIAA1122; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1; ATP-dependent helicase 1; hHEL1
Host
Rabbit
Reactivity
H, M
Immunogen
Recombinant full length protein of human SMARCAD1
Clonality
Polyclonal
Applications
WB, IF/IC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/100), IP (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of SMARCAD1 expression in A549 (A), HepG2 (B), mouse kidney (C), mouse lung (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of SMARCAD1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
56916
Gene ID (Mouse)
13990
SwissProt (Human)
Q9H4L7
SwissProt (Mouse)
Q04692
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