Rabbit anti-SMAD3 (pS425) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SMAD3 (pS425)
Synonyms
MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Host
Rabbit
Reactivity
H, C
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SMAD3 (pS425) . The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of SMAD3 (pS425) expression in A431 EGF-treated (A), HL60 TGF-treated (B) whole cell lysates.
IHC description
Immunohistochemical analysis of SMAD3 (pS425) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4088
SwissProt (Human)
P84022
CAS Number
9007-83-4
Available Sizes
Curated Selection
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