Rabbit anti-SMAD3 (pS425) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to SMAD3 (pS425)

CAS Number

9007-83-4

Synonyms

MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Host

Rabbit

Reactivity

H, C

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SMAD3 (pS425) . The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of SMAD3 (pS425) expression in A431 EGF-treated (A), HL60 TGF-treated (B) whole cell lysates.

IHC description

Immunohistochemical analysis of SMAD3 (pS425) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.