Rabbit anti-SMAC Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SMAC
Synonyms
SMAC; Diablo homolog, mitochondrial; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; Smac
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human SMAC
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of SMAC expression in SW620 (A), BT474 (B), mouse spleen (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of SMAC staining in SW620 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
56616
Gene ID (Mouse)
66593
SwissProt (Human)
Q9NR28
SwissProt (Mouse)
Q9JIQ3
CAS Number
9007-83-4
Available Sizes
Curated Selection
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