Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-SGK1/2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to SGK1/2

Synonyms

SGK1; SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1; SGK2; Serine/threonine-protein kinase Sgk2; Serum/glucocorticoid-regulated kinase 2

Host

Rabbit

Reactivity

H, M, R, B, C, Rb, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SGK1/2. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of SGK1/2 expression in Jurkat (A), HeLa (B), NIH3T3 (C), PC12 (D) whole cell lysates.

IHC description

Immunohistochemical analysis of SGK1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of SGK1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

6446; 10110

Gene ID (Mouse)

20393; 27219

Gene ID (Rat)

171497

SwissProt (Human)

O00141; Q9HBY8

SwissProt (Mouse)

Q9WVC6; Q9QZS5

SwissProt (Rat)

Q06226; Q8R4U9

CAS Number

9007-83-4

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

LOC283685 ORF Vector (Human) (pORF)
27059011 1.0 µg DNA

LOC283685 ORF Vector (Human) (pORF)

Ask
View Details
PAR4 Rabbit pAb (APR25099N)
APR25099N-01 50 µL

PAR4 Rabbit pAb (APR25099N)

Ask
View Details
PAR4 Rabbit pAb (APR25099N)
APR25099N-02 100 µL

PAR4 Rabbit pAb (APR25099N)

Ask
View Details
PAR4 Rabbit pAb (APR25099N)
APR25099N-03 200 µL

PAR4 Rabbit pAb (APR25099N)

Ask
View Details
Rsk-2 Lentiviral Activation Particles (h)
sc-401003-LAC 200 µL

Rsk-2 Lentiviral Activation Particles (h)

Ask
View Details
Cas scaffolding protein family member 4 (CASS4) Mouse ELISA Kit
C1519 96 Tests

Cas scaffolding protein family member 4 (CASS4) Mouse ELISA Kit

Ask
View Details