What is the catalog number for Rabbit anti-SF2 Antibody?

The catalog number for Rabbit anti-SF2 Antibody is YLD7114.

What sizes are available for Rabbit anti-SF2 Antibody?

Rabbit anti-SF2 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-SF2 Antibody come in?

Rabbit anti-SF2 Antibody is available in 2 different pack sizes.

Is Rabbit anti-SF2 Antibody in stock?

Rabbit anti-SF2 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-SF2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to SF2

CAS Number

9007-83-4

Synonyms

ASF; SF2; SF2P33; SFRS1; Serine/arginine-rich splicing factor 1; Alternative-splicing factor 1; ASF-1; Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human SF2

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of SF2 expression in MCF7 (A), A549 (B), mouse spleen (C) whole cell lysates.

IHC description

Immunohistochemical analysis of SF2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of SF2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.