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Rabbit anti-SerpinB1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to SerpinB1

Synonyms

ELANH2; MNEI; PI2; Leukocyte elastase inhibitor; LEI; Monocyte/neutrophil elastase inhibitor; EI; M/NEI; Peptidase inhibitor 2; PI-2; Serpin B1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human SerpinB1

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of SerpinB1 expression in MCF7 (A), A549 (B), mouse lung (C), mouse spleen (D), rat spleen (E) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of SerpinB1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

1992

SwissProt (Human)

P30740

CAS Number

9007-83-4

Available Sizes

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