Rabbit anti-SERCA1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SERCA1
Synonyms
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca (2+) -ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca (2+) ATPase
Host
Rabbit
Reactivity
H, M, R, B, Rb
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SERCA1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of SERCA1 expression in A549 (A), mouse muscle (B), rat muscle (C) whole cell lysates.
IHC description
Immunohistochemical analysis of SERCA1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
487
Gene ID (Mouse)
11937
Gene ID (Rat)
116601
SwissProt (Human)
O14983
SwissProt (Mouse)
Q8R429
SwissProt (Rat)
Q64578
CAS Number
9007-83-4
Available Sizes
Curated Selection
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