Rabbit anti-SAP145 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SAP145
Synonyms
SAP145; Splicing factor 3B subunit 2; Pre-mRNA-splicing factor SF3b 145 kDa subunit; SF3b145; SF3b150; Spliceosome-associated protein 145; SAP 145
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human SAP145
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of SAP145 expression in Hela (A), MCF7 (B), mouse testis (C) whole cell lysates.
IHC description
Immunohistochemical analysis of SAP145 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
10992
SwissProt (Human)
Q13435
CAS Number
9007-83-4
Available Sizes
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