Rabbit anti-SAP14 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SAP14
Synonyms
SAP14; SF3B14; SF3B14A; Splicing factor 3B subunit 6; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit; SF3B14a; Spliceosome-associated protein, 14-kDa; Splicing factor 3b, subunit 6,14kDa
Host
Rabbit
Reactivity
H, M, R, B, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SAP14. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of SAP14 expression in HepG2 (A), RT4 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of SAP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
51639
Gene ID (Mouse)
66055
SwissProt (Human)
Q9Y3B4
SwissProt (Mouse)
P59708
CAS Number
9007-83-4
Available Sizes
Curated Selection
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