Rabbit anti-RPS10 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to RPS10
CAS Number
9007-83-4
Synonyms
40S ribosomal protein S10
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human RPS10
Clonality
Polyclonal
Applications
WB, IHC, IF/IC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/20 - 1/50), IP (1/20 - 1/50)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of RPS10 expression in A549 (A), HT29 (B), mouse spleen (C), mouse heart (D) whole cell lysates.
IHC description
Immunohistochemical analysis of RPS10 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of RPS10 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
6204
Gene ID (Mouse)
67097
Gene ID (Rat)
100363439; 81773
SwissProt (Human)
P46783
SwissProt (Mouse)
P63325
SwissProt (Rat)
P63326
Available Sizes
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