Rabbit anti-RPL35 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to RPL35
Synonyms
60S ribosomal protein L35
Host
Rabbit
Reactivity
H, M, R, B, C, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPL35. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of RPL35 expression in U2OS (A), HeLa (B), NIH3T3 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of RPL35 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
11224
Gene ID (Mouse)
66489
Gene ID (Rat)
296709
SwissProt (Human)
P42766
SwissProt (Mouse)
Q6ZWV7
SwissProt (Rat)
P17078
CAS Number
9007-83-4
Available Sizes
Curated Selection
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