Rabbit anti-ROCK1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ROCK1
Synonyms
Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK
Host
Rabbit
Reactivity
H, M, R, B, Mk, P, Rb
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ROCK1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ROCK1 expression in HeLa (A), NIH3T3 (B), C6 (C), COS7 (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of ROCK1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
6093
Gene ID (Mouse)
19877
Gene ID (Rat)
81762
SwissProt (Human)
Q13464
SwissProt (Mouse)
P70335
SwissProt (Rat)
Q63644
CAS Number
9007-83-4
Available Sizes
Curated Selection
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