Rabbit anti-RING1b Antibody

Product Specifications

Background

Rabbit polyclonal antibody to RING1b

CAS Number

9007-83-4

Synonyms

BAP1; DING; HIPI3; RING1B; E3 ubiquitin-protein ligase RING2; Huntingtin-interacting protein 2-interacting protein 3; HIP2-interacting protein 3; Protein DinG; RING finger protein 1B; RING1b; RING finger protein 2; RING finger protein BAP-1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human RING1b

Clonality

Polyclonal

Applications

WB, IHC, IF/IC, IP, ChIP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/50 - 1/200), ChIP (1/20 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of RING1b expression in Jurkat (A), SHSY5Y (B), mouse testis (C) whole cell lysates.

IHC description

Immunohistochemical analysis of RING1b staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of RING1b staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.