Rabbit anti-RhoT1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to RhoT1
Synonyms
ARHT1; Mitochondrial Rho GTPase 1; MIRO-1; hMiro-1; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human RhoT1
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of RhoT1 expression in A549 (A), mouse brain (B), mouse kidney (C) whole cell lysates.
IHC description
Immunohistochemical analysis of RhoT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
55288
Gene ID (Mouse)
59040
SwissProt (Human)
Q8IXI2
SwissProt (Mouse)
Q8BG51
CAS Number
9007-83-4
Available Sizes
Curated Selection
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