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Rabbit anti-RBAT Antibody

Product Specifications

Background

Rabbit polyclonal antibody to RBAT

CAS Number

9007-83-4

Synonyms

RBAT; Neutral and basic amino acid transport protein rBAT; NBAT; D2h; Solute carrier family 3 member 1; b (0, +) -type amino acid transport protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human RBAT

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of RBAT expression in HT29 (A), HepG2 (B), PC12 (C), mouse liver (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of RBAT staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

6519

Gene ID (Mouse)

20532

Gene ID (Rat)

29484

SwissProt (Human)

Q07837

SwissProt (Mouse)

Q91WV7

SwissProt (Rat)

Q64319

Available Sizes

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