Rabbit anti-RagA/B Antibody
Product Specifications
Background
Rabbit polyclonal antibody to RagA/B
Synonyms
RRAGA; Ras-related GTP-binding protein A; Rag A; RagA; Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1; RRAGB; Ras-related GTP-binding protein B; Rag B; RagB
Host
Rabbit
Reactivity
H, M, R, B, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RagA/B. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of RagA/B expression in MCF7 (A), HeLa (B), mouse brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of RagA/B staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
10670; 10325
Gene ID (Mouse)
68441; 245670
Gene ID (Rat)
117044; 100909655
SwissProt (Human)
Q7L523; Q5VZM2
SwissProt (Mouse)
Q80X95; Q6NTA4
SwissProt (Rat)
Q63486; Q63487
CAS Number
9007-83-4
Available Sizes
Curated Selection
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