Rabbit anti-RAD9A Antibody
Product Specifications
Background
Rabbit polyclonal antibody to RAD9A
Synonyms
RAD9A; Cell cycle checkpoint control protein RAD9A; hRAD9; DNA repair exonuclease rad9 homolog A
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human RAD9A
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of RAD9A expression in mouse testis (A) whole cell lysates.
IHC description
Immunohistochemical analysis of RAD9A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of RAD9A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5883
Gene ID (Mouse)
19367
SwissProt (Human)
Q99638
SwissProt (Mouse)
Q9Z0F6
CAS Number
9007-83-4
Available Sizes
Curated Selection
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